onix observation chamber b04a-03-5pk cellasic onix plates for bacteria cells  (CellASIC Corporation)

Journal: bioRxiv

Article Title: Deep learning-driven imaging of cell division and cell growth across an entire eukaryotic life cycle

doi: 10.1101/2024.04.25.591211

Figure Lengend Snippet: (A) Schematic of S. cerevisiae life cycle. F0 = parental generation, F1 = filial generation 1 (B) Schematic microfluidic protocol to specifically trigger S. cerevisiae’s sexual life cycle by selecting against non-sporulated cells that can outcompete germinating spores. Nuclei represented as solid shapes inside the cells. (C-D) Representative time lapse micrographs of computationally aligned yeast cells undergoing (C) homothallic life cycle type A, in which sporulation is followed by ascus mating directly leading to two diploid cells without an intervening proliferative haploid state, or (D) Homothallic life cycle type B, in which sporulation is followed by partial ascus mating producing one diploid and two proliferative haploid cells which eventually diploidize through inbreeding.

Article Snippet: The culture was spun down on a tabletop microfuge for 3 seconds to remove clumps, and 70 μl of the top layer were transferred to a microfluidics device with flow control (Y04C CellASIC plate with OniX controllers), pre-warmed at 25 °C.

Techniques:

Journal: bioRxiv

Article Title: Deep learning-driven imaging of cell division and cell growth across an entire eukaryotic life cycle

doi: 10.1101/2024.04.25.591211

Figure Lengend Snippet: (A) Schematic microfluidic induction of sequential S. cerevisiae sexual life cycles. Solid circles = nuclei; green = LiCH, orange = Whi5-mSC. (B) Representative scaled average size, Whi5-mSC or LiCHI nuclear concentration during the transition from cell birth into the first mitotic division aligned to the peak of Whi5 concentration during G1 (C-E) Boxplot comparison of the first mitotic division in three sexually reproducing generations (F0-F2) in terms of (C) cell size, (D) peak nuclear Whi5 levels, (E) total Whi5 cell concentration. (F-H) Boxplot comparison of the kinetics of meiotic divisions in three sexually reproducing generations (F0-F2) in terms of the duration between the time point of anaphase I and (F) pre-meiotic G1 exit, (G) prophase I exit, (H) meiotic exit. (I-K) Correlation between the time of anaphase I and pre-meiotic G1 exit in (I) F0, (J) F1, and (K) F2 generations. (L-N) Correlation between the time of anaphase I and cell size at anaphase I (L) F0, (M) F1, and (N) F2 generations.

Article Snippet: The culture was spun down on a tabletop microfuge for 3 seconds to remove clumps, and 70 μl of the top layer were transferred to a microfluidics device with flow control (Y04C CellASIC plate with OniX controllers), pre-warmed at 25 °C.

Techniques: Concentration Assay, Comparison